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nanopore genome sequencing  (ATCC)


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    ATCC nanopore genome sequencing
    Nanopore Genome Sequencing, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanopore genome sequencing/product/ATCC
    Average 94 stars, based on 30 article reviews
    nanopore genome sequencing - by Bioz Stars, 2026-03
    94/100 stars

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    Mitochondrial genome organization and transcriptional activity in T. penetrans . A) A circular plot depicting the genomic organization of the complete mitochondrial genome of human parasite, Tunga penetrans . The mitochondrial genome encodes 13 protein coding genes, 22 transfer RNAs and 2 ribosomal RNA genes, along with a replication origin. Genes are color-coded by functional category and transcribed in clockwise (outer circle) or counterclockwise (inner circle) directions. The genome’s organization and gene order are conserved relative to other Siphonaptera species. B) RNA-Seq read coverage across the mitochondrial genome of T. penetrans . The coverage plot highlights regions of high transcriptional activity, particularly in the large ribosomal RNA subunit ( rrnL ) and cytochrome c oxidase genes ( cox1-cox3 ). RNA-Seq reads were mapped to the mitochondrial genome to validate and refine the assembly, ensuring accuracy in gene annotation. The red line indicates number of sequences from the <t>nanopore</t> reads that mapped back to the genome.
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    Mitochondrial genome organization and transcriptional activity in T. penetrans . A) A circular plot depicting the genomic organization of the complete mitochondrial genome of human parasite, Tunga penetrans . The mitochondrial genome encodes 13 protein coding genes, 22 transfer RNAs and 2 ribosomal RNA genes, along with a replication origin. Genes are color-coded by functional category and transcribed in clockwise (outer circle) or counterclockwise (inner circle) directions. The genome’s organization and gene order are conserved relative to other Siphonaptera species. B) RNA-Seq read coverage across the mitochondrial genome of T. penetrans . The coverage plot highlights regions of high transcriptional activity, particularly in the large ribosomal RNA subunit ( rrnL ) and cytochrome c oxidase genes ( cox1-cox3 ). RNA-Seq reads were mapped to the mitochondrial genome to validate and refine the assembly, ensuring accuracy in gene annotation. The red line indicates number of sequences from the <t>nanopore</t> reads that mapped back to the genome.
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    Mitochondrial genome organization and transcriptional activity in T. penetrans . A) A circular plot depicting the genomic organization of the complete mitochondrial genome of human parasite, Tunga penetrans . The mitochondrial genome encodes 13 protein coding genes, 22 transfer RNAs and 2 ribosomal RNA genes, along with a replication origin. Genes are color-coded by functional category and transcribed in clockwise (outer circle) or counterclockwise (inner circle) directions. The genome’s organization and gene order are conserved relative to other Siphonaptera species. B) RNA-Seq read coverage across the mitochondrial genome of T. penetrans . The coverage plot highlights regions of high transcriptional activity, particularly in the large ribosomal RNA subunit ( rrnL ) and cytochrome c oxidase genes ( cox1-cox3 ). RNA-Seq reads were mapped to the mitochondrial genome to validate and refine the assembly, ensuring accuracy in gene annotation. The red line indicates number of sequences from the <t>nanopore</t> reads that mapped back to the genome.
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    Mitochondrial genome organization and transcriptional activity in T. penetrans . A) A circular plot depicting the genomic organization of the complete mitochondrial genome of human parasite, Tunga penetrans . The mitochondrial genome encodes 13 protein coding genes, 22 transfer RNAs and 2 ribosomal RNA genes, along with a replication origin. Genes are color-coded by functional category and transcribed in clockwise (outer circle) or counterclockwise (inner circle) directions. The genome’s organization and gene order are conserved relative to other Siphonaptera species. B) RNA-Seq read coverage across the mitochondrial genome of T. penetrans . The coverage plot highlights regions of high transcriptional activity, particularly in the large ribosomal RNA subunit ( rrnL ) and cytochrome c oxidase genes ( cox1-cox3 ). RNA-Seq reads were mapped to the mitochondrial genome to validate and refine the assembly, ensuring accuracy in gene annotation. The red line indicates number of sequences from the nanopore reads that mapped back to the genome.

    Journal: bioRxiv

    Article Title: The complete mitochondrial genome of Tunga penetrans and insights into flea phylogeny

    doi: 10.1101/2025.06.09.658575

    Figure Lengend Snippet: Mitochondrial genome organization and transcriptional activity in T. penetrans . A) A circular plot depicting the genomic organization of the complete mitochondrial genome of human parasite, Tunga penetrans . The mitochondrial genome encodes 13 protein coding genes, 22 transfer RNAs and 2 ribosomal RNA genes, along with a replication origin. Genes are color-coded by functional category and transcribed in clockwise (outer circle) or counterclockwise (inner circle) directions. The genome’s organization and gene order are conserved relative to other Siphonaptera species. B) RNA-Seq read coverage across the mitochondrial genome of T. penetrans . The coverage plot highlights regions of high transcriptional activity, particularly in the large ribosomal RNA subunit ( rrnL ) and cytochrome c oxidase genes ( cox1-cox3 ). RNA-Seq reads were mapped to the mitochondrial genome to validate and refine the assembly, ensuring accuracy in gene annotation. The red line indicates number of sequences from the nanopore reads that mapped back to the genome.

    Article Snippet: The high molecular weight DNA was used to prepare the Nanopore Rapid sequencing genomic DNA libraries and subsequently sequenced using the Oxford Nanopore MinION platform.

    Techniques: Activity Assay, Functional Assay, RNA Sequencing